A new dimension in selectivity with SelexION® DMS
Differential mobility spectrometry (DMS) and ion mobility spectrometry are analytical techniques used to separate hard to resolve ions based on their gas-phase mobility. Multiple types of ion mobility devices exist, such as drift tubes, traveling wave, and high-field asymmetric waveform devices. However, none carry the power of SCIEX SelexION®Technology that delivers highly-selective, robust and powerful ion separation that significantly enhances your quantitative and qualitative performance.
The SelexION®Differential Mobility Device can help you address your biggest analytical challenges:
- Overcome co-eluting matrix interferences and improve data quality in complex samples
- Separate isobaric compounds for increased confidence in detection
- Detect and quantify isobaric lipid molecular species for more accurate biological insights
- Reduce background noise that may be limiting your LOQ
- Save time with simplified sample preparation
The power of SelexION takes you beyond limitation
Overcome your most advanced analytical challenges
For scientists who are challenged by assays with isobaric interferences and difficult-to-separate co-eluting contaminants, this fast, reproducible and easy-to-use approach will enhance the selectivity of your LC-MS/MS separations.
Save time
Enhance selectivity without the need for additional complex sample preparation
Fast separation
Perform fast separation analyses compatible with UHPLC timescales
Improve LOQ
Overcome co-eluting matrix interferences and improve data quality in complex samples
Boost confidence
Separate and quantify isobaric molecular species for more accurate biological insights
2-minute install
Maximize mass spec workflow flexibility with easy install that doesn’t require breaking the system vacuum
The science behind unmatched separation
SCIEX SelexION device is a small, planar mobility cell that significantly increases analytical separation power, even if compounds have identical molecular weights and chromatographic retention times, made possible by a mobility region and optional polar modifiers in the transport region.
Mobility separation
Two parallel flat plates define a mobility region, allowing nitrogen carrier gas to create forward directional flow between the ion source and the mass spec analyzer. Analytes are separated based on their respective size, shape, charge state and chemical interaction, prior to entering the mass spec.
Modifier separation
Polar modifiers in the transport region can lead to a significant increase in analyte separation and peak capacity. Chemical modifiers, such as isopropanol or acetonitrile, influence ion mobility based on their ability to cluster with analytes.
SelexION in action
See how the small, planar SelexION DMS device can enhance analytical separations for isobaric species and co-eluting contaminants.
A step above the rest: The better way to see more
SelexION offers a unique approach to separation that is unmatched by any other available method. Firstly, it is a planar device that separates ions based on differences in mobility in two different regions of the field dependent mobility curve (Ref1). Secondly, due to its size, the system can operate with very short residence times with optimal performance when using chemical modifiers, and can be used in transparent mode to allow for maximal workflow flexibility.
Enhanced isobaric separation
Other types of standard low field mobility devices, such as drift tubes, separate ions based upon differences in the low field mobility constant related to analyte mass, and are generally less effective at separating isobaric ions (Refs2,3).
Planar resolving power
Planar devices such as SelexION eliminates heterogeneous electric fields within the device, leading to increased resolving power compared to curved surface mobility devices (Refs4,5).
Separation flexibility
SelexION offers the ability to turn off separation voltage and operate in a ‘transparent’ mode to transmit all ions, providing the option to perform non-ion mobility assays without removing the front-end device (Ref1).
SelexION applications and workflows
Bring the power of SelexION Technology to your application
From pharmaceutical drug discovery/development and lipidomic analysis to food, environmental testing and so much more, learn how SelexION technology has enhanced results in these key applications.
Lipidomic analysis
Improve lipid class separation for more confident lipid ID
Novel lipid screening
Get a complete solution for lipidomics research
Bioanalytical quantitation
Improve selectivity for quantitative bioanalysis
Food contaminants
Identify and quantify food contaminants
Biological matrices
Successful separation of diastereomeric monohydroxy Flubatine standards
Get more from your mass spec
References:
- Schneider, B. B., Nazarov, E. G., Londry, F., Vouros, P. and Covey, T. R. (2016), Differential mobility spectrometry/mass spectrometry history, theory, design optimization, simulations, and applications. Mass Spec Rev, 35: 687–737.
- Shvartsburg AA, Prior DC, Tang K, Smith RD, “High-Resolution differential Ion Mobility Separations Using Planar Analyzers at Elevated Dispersion Fields”, Anal. Chem., 2010, 82, 7649-7655.
- Revercomb and Mason, “Theory of plasma chromatography/gaseous electrophoresis”, Anal. Chem., 47, 1975, 970-983.
- Shvartsburg AA, Li F, Tang K, Smith RD, “High-Resolution Field Asymmetric Waveform Ion Mobility Spectrometry Using New Planar Geometry Analyzers”, Anal. Chem., 2006, 78, 3706-3714.
- Krylov EV, Nazarov EG, Miller RA, “Differential Mobility Spectrometer: Model of Operation”, Int. J. Mass Spectrom., 266, 2007, 76-85.
